Since 1993, D'Abruzzo has performed as various Muppets in Sesame Street, including a notable performance singing with R.E.M. in a new version of their song "Shiny Happy People" called "Furry Happy Monsters".
D'Abruzzo has performed in developmental readings of Avenue Q, I Love You Because, Oh, What a Lovely War!, The $trip, The Medium at Large, and The Green Room. She has also been featured in several concerts and benefits, including Skitch Henderson's New Faces of 2004 at Carnegie Hall, Encores! 10th Anniversary Bash, Chess (for the Actors' Fund), Children and Art: Stephen Sondheim's 75th Birthday Gala, and Stephen Sondheim's 75th: The Concert (at the Hollywood Bowl).
In May 2005, D'Abruzzo made her solo cabaret debut at the New York City jazz club Birdland.
Her other puppeteering and voice work includes appearances in Oobi, Pinky Dinky Doo, Sheep in the Big City, The Book of Pooh, The Wubbulous World of Dr. Seuss, and Jack's Big Music Show, along with various commercials and promos. She guest starred in a musical episode of Scrubs titled "My Musical", as a patient with a mysterious ailment that caused her to interpret speech as Broadway-esque song and dance numbers. She sang in five of the nine songs. Four of the episode's songs were co-written by Avenue Q composers Robert Lopez and Jeff Marx. The episode aired on January 18, 2007 and was nominated for five Emmy Awards. She later appeared in a cameo in episode "My Finale", as series protagonist J.D. leaves Sacred Heart for the final time.
D'Abruzzo appears on the original cast recordings of Avenue Q and I Love You Because and performs the parts of "Sheldon" and "Deb" on the studio recording of Finding Nemo – The Musical, a new musical production performed several times each day at Disney's Animal Kingdom. She is also one of the performers included in the documentary Showbusiness: The Road to Broadway, which chronicles the 2003-2004 Broadway season.
D'Abruzzo confirmed on her official website that for Sesame Street season 46, she will be performing Prairie Dawn, following the retirement of Prairie Dawn's original performer, Fran Brill on October 2, 2014.