She was married to actor James McCallion from 1943 until her death in 1977. She guest starred in the NBC family drama, National Velvet, starring Lori Martin, Ann Doran, and Arthur Space. Her husband was a National Velvet series regular, cast as the ex-jockey Mi Taylor. In addition to National Velvet, she and McCallion appeared together in Wagon Train and The Big Valley. Their son, Denis McCallion, became a television Producer.
She appeared twice on Rod Cameron syndicated series, State Trooper, as Julia Brundidge in "Meeting at Julias" (1956) and as Sarah Brinkman in "The Clever Man" (1958). She then appeared in 1960 in Cameron's other crime drama series, Coronado 9, as Nora Morgan in the episode "Run Scared." From 1973-77 she had a recurring role on The Waltons as boarding house landlady Flossie Brimmer.
Marlowe was cast in the 1959-1960 television season as Martha Commager, the owner of a boarding house, in seven episodes of the NBC western series, Law of the Plainsman. She appeared three times as Mrs. Moffatt on the CBS sicom My Living Doll, starring Robert Cummings and Julie Newmar.
Marlowe appeared seven times on Wagon Train, six times on Gunsmoke, and twice on Dick Powell's Zane Grey Theater. Her many other guest-starring roles included Schlitz Playhouse, 77 Sunset Strip, Hawaiian Eye, The Millionaire, Shotgun Slade, Hotel de Paree, General Electric Theater, 87th Precinct, Frontier Circus, The Alfred Hitchcock Hour, The Donna Reed Show, Petticoat Junction, Going My Way, Twelve O'Clock High, Family Affair, The Green Hornet, Lassie, Bridget Loves Bernie, Here Come the Brides, Barnaby Jones, Medical Center, Cade's County, Cannon, The Rockford Files, The Big Valley, The Guns of Will Sonnett, The F.B.I., Marcus Welby, M.D., The Outer Limits, The Bob Newhart Show, The Streets of San Francisco, and most notably her two appearances on The Twilight Zone: the 1961 episode "Back There" and the 1964 episode "Night Call".
Born in Worcester, Massachusetts, Marlowe was best known for her role from 1973 to 1977 as Flossie Brimmer in twenty-seven episodes of the CBS family drama, The Waltons.
Marlowe died in Los Angeles, California on December 31, 1977.