Hoffmann was born in Switzerland on 24 October 1868, and died on 18 April 1920.
In 1894 he formed a company with Max Carl Traub (1855–1919) called Hoffmann, Traub & Co., which manufactured a limited range of pharmaceutical and chemical products. His Father contributed the majority of the capital, Traub some patents and contracts. In 1896 Traub left the company and it was renamed F. Hoffmann-La Roche & Co..
On 2 May 1895 Fritz Hoffmann married Adèle La Roche (1876–1938). It was a Common practice in Switzerland for married couples to hyphenate the name, so from this point he was often referred to as Fritz Hoffmann-La Roche. They had two sons, Emmanuel (4 May 1896, Basel – 3 October 1932, Basel) and Alfred (25 July 1898–1987). The couple were divorced in 1919 and he married divorcée Elisabeth von der Mühll (1882–1970).
According to Peyer (See below) "For his time, Fritz Hoffmann was unusually interested in product promotion" and paid attention to all aspects of publicity from simple advertising to packaging and promotion of key individuals such as pharmacists. He also built up international contacts for raw materials and product sales, and supported research. Following the setback of the 1st World War, these factors helped the company to recover and expand. He retired from the board of the company because of ill-health in 1919 and died the following year.